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. Author manuscript; available in PMC: 2012 Jul 11.
Published in final edited form as: J Neurosci. 2012 Jan 11;32(2):583–592. doi: 10.1523/JNEUROSCI.2849-11.2012

Figure 5. Downregulation of endogenous PHB by RNAi increases neuronal vulnerability.

Figure 5

(A) si-PHB-2 downregulates endogenous PHB expression, while si-control and si-PHB-1 have no effect. (B) si-PHB-2 decreases PHB protein band intensity by more than 75% (*p<0.05; n=4). (C) “Knockdown” of PHB by si-PHB-2 enhances the increase in caspase-3 activity induced by STS compared to si-Ctrl and si-PHB-1(*p<0.05 from respective vehicle; # p<0.05 from si-PHB-1 and si-Ctrl; n=6/group). (D) si-PHB-2 reduces the viability of neuronal cultures exposed to X-XO, assessed by trypan blue exclusion (*p<0.05 from si-PHB-1; n=4/group, 2500 cells/group). (E) si-PHB2 enhances the decrease in relative membrane potential (JC1 fluorescence ratio) induced by X-XO (*p<0.05; n=5).