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. Author manuscript; available in PMC: 2012 Mar 7.
Published in final edited form as: Mol Plant Pathol. 2011 Mar 1;12(8):746–758. doi: 10.1111/j.1364-3703.2011.00708.x

Figure 3.

Figure 3

Subcellular localization of EDR1. Panels a–c, EDR1-sYFP and GCN5-mCherry were transiently co-expressed in N. benthamiana leaves and imaged using confocal laser scanning microscopy. Panel a, EDR1-sYFP (a single optical section taken through the nucleus of an epidermal cell). Panel b, GCN5-mCherry expressed in the same cell. Panel c, overlay of panels a and b. Panels d–f, EDR1-sYFP and an mCherry ER marker (see Methods) were transiently co-expressed in N. benthamiana. d, EDR1-sYFP (a single optical section taken through the cell cortex of an epidermal cell); e, mCherry-HDEL, f, overlay of d and e. Panel g, immunoblot of EDR1-sYFP extracted from N. benthamiana leaves. KEG-sYFP is an unrelated YFP fusion protein included to show specificity of the antibody. Scale bar is 25 µm.