Figure 8. Knockdown of PXR and derepression of UGT1A1 gene expression in primary hepatocytes.

Hepatocytes were isolated from 14 day-old neonatal hUGT1*1 mice. Cells were transfected with mouse siRNA oligonucleotide manufactured by Bioneer (siPXR_b) and Santa Cruz Biotechnology (siPXR_sc). Forty eight hours later, cells were harvested for RNA preparation. The relative gene expression levels of mouse Pxr, human UGT1A1, and mouse Cyp3a11 were determined by RT and Q-PCR (** p<0.01, t-test).