Skip to main content
. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Influenza Other Respir Viruses. 2012 Apr 12;7(1):85–92. doi: 10.1111/j.1750-2659.2012.00358.x

Table 3.

RT-PCR analysis of oral swabs from ruddy turnstones experimentally inoculated with influenza isolate A/shorebird/DE/42/2006 (H7N3).

Bird ID Dose1 DPI 0 DPI 1 DPI 2 DPI 3 DPI 4 DPI 5 DPI 6 DPI 7 DPI 9
61 3.25 -2 - 32.5c 38.49c - - - - -
683 3.25 - - - - - - - - -
63 3.25 - - - - - - - - -
12 3.25 - - - - - - - - -
13 3.25 - - - - - - - - -
14 3.25 - - - - - - - - -
3 3.75 - 27.77* 34.53* - - - - - -
10 3.75 - - - - - - - - -
55 3.75 - - - - - - - - -
15 3.75 - 29.24 33.87 39.95 - - - - -
13 3.75 - 39.43 32.3 - - - - - -
14 3.75 - 29.68* 32.91 34.08 38.06* 32* 35 39.43 -
68 3.75 - - - 35.77 - - - - -
59 3.75 - 31.51* 30.43* 31.33* 29.48* 28.07* 27.59* 32.86* 39.48*
4 3.75 - 33.83* 33.45* 40.62 - - - - -
69 3.75 - 35.92* 38.08* -* 38.01 - - - -
60 4.75 - 30.7* 34.21 - 39.97 40.14 - - -
80 4.75 - 37.31 - 40.34 - - - - -
57 4.75 - - 39.25 37.05 - - - - -
58 4.75 - 32.82 32.99* 35.72 31.98* 28.52* 39.24 - -
153 5.25 - - - - - - - - -
18 5.25 - 29.19*c 31.93c 34.34c 38.85c 40.25c - - -
64 5.25 - 30.71*c 36.82c 39.31c - - - - -
19 5.25 - 29.67*c 32.71*c 35.28c 38.25*c - - - -
17 5.25 - 28.48*c 28.58c 29.95*c 34.01c 39.98c - - -
65 5.25 - 30.87*c 25.9*c 27.37*c 27.94*c 27.14*c 33.07*c 34.29c 37.16*c
1

Inocula doses expressed as Log10EID50/100µl. No bird inoculated with lower viral titers became infected based on viral excretion and seroconversion except bird 29 that seroconverted but did not shed viral RNA (not shown). This bird was housed long-term to determine the stability of antibody response.

2

Ct values are from RT-PCR analyses using avian influenza H7 specific primers and probe. Samples with no Ct values are shown with -.

c

Indicates that RT-PCR positive cloacal swabs were detected on the DPI shown.

3

These birds were uninfected and remained seronegative from the first challenge round and were reused in a second challenge with this virus isolate.

*

Presence of viable virus confirmed by virus isolation in embryonating egg culture.