Table 4.
RT-PCR analysis of oral swabs from ruddy turnstones experimentally inoculated with influenza isolate A/northern pintail/California/44242-758/2006 (H5N2).
| Bird ID | Dose1 | DPI 0 | DPI 1 | DPI 2 | DPI 3 | DPI 4 | DPI 5 | DPI 6 | DPI 7 | DPI 9 |
|---|---|---|---|---|---|---|---|---|---|---|
| 5 | Control | -2 | - | - | - | - | - | - | - | - |
| 70 | Control | - | - | - | - | - | - | - | - | - |
| 7 | 2 | - | - | - | - | - | - | - | - | - |
| 9 | 2 | - | 38.093 | - | - | - | - | - | - | - |
| 11 | 2 | - | - | - | - | - | - | - | - | - |
| 16 | 2 | - | - | - | - | - | - | - | - | - |
| 20 | 2 | - | 39.513 | - | - | -c | - | -c | - | - |
| 51 | 2 | - | 38.233 | - | - | - | - | - | - | - |
| 52 | 4 | - | - | - | - | - | - | - | - | - |
| 59 | 4 | - | - | - | - | - | - | - | - | - |
| 53 | 4 | - | - | 37.6 | 37.85* | 32.46* | - | -c | - | - |
| 54 | 4 | - | - | - | - | - | - | - | - | - |
| 67 | 4 | - | 30.1* | 30.08* | 34.18c | - | - | - | - | - |
| 66 | 4 | - | - | 33.85 | 33.99 | - | 37.82 | - | - | - |
| 57 | 6 | - | - | - | - | 39.79 | 37.56 | - | - | - |
| 58 | 6 | - | 34.95 | 37.21 | 36.26 | 34.64 | 38.24 | - | - | - |
| 60 | 6 | - | 33.85 | 35.2 | 38.63 | - | - | - | - | - |
| 62 | 6 | - | 37.11 | 35.4 | 32.55 | - | - | 38.93 | - | 39.7 |
| 4 | 6 | - | 30.89c | 32.1 | 35.54 | - | - | - | - | - |
| 69 | 6 | - | 30.8c | 36.64 | - | - | - | - | - | - |
1
Inocula doses expressed as Log10EID50/100µl. No bird inoculated with lower viral titers became infected based on viral excretion and seroconversion (not shown).
2
Ct values are from RT-PCR analyses using avian influenza H5 specific primers and probe. Samples with no Ct values are shown with -.
c
Indicates that RT-PCR positive cloacal swabs were detected on the DPI shown.
3
The presence of viral RNA detected on DPI1 only was assumed to be residual inoculum.
*
Presence of viable virus confirmed by virus isolation in embryonating egg culture.