. Author manuscript; available in PMC: 2014 Jan 1.

Published in final edited form as: Influenza Other Respir Viruses. 2012 Apr 12;7(1):85–92. doi: 10.1111/j.1750-2659.2012.00358.x

Table 5.

RT-PCR analysis of oral swabs from ruddy turnstones experimentally inoculated with highly pathogenic avian influenza isolate A/whooperswan/Mongolia/244/05 (H5N1).

Bird ID	Dose¹	DPI 0	DPI 1	DPI 2	DPI 3	DPI 4	DPI 5	DPI 6	DPI 7	DPI 8	DPI10
67	Control	-²	-	-	-	-	-	-	-	-	-
79	Control	-	-	-	-	-	-	-	-	-	-
64	4.25	-	26.9^*	33.46	36.38	-	38.75	-^c	-	-	-
12	4.25	-	31.26	28.01^*	34.55	33.37	27.83^*	31.49	37.13	-	38.21
63	4.25	-	-	-	30.31^*	31.13	32.51^*^c	33.81	36.59	34.79	38.09
8	4.25	-	-	-	-	38.66^*	-	-	-	39.89	-
6	4.25	-	29.97	30.05	33.29^c	33.56^c	29.28^c	28.81	31.86^*	34.84	-
30	4.25	-	-	37.36	-	-	-	-	-	-	-
54	5.25	-	37.28	34.12	-	39.49	-	-	-	-	-
65	5.25	-	-	-	-	37.44	-	-	-	-	-
52	5.25	-	31.75	37.04	-	-	-	-	-	-	-
51	5.25	-	36.75	36.61	38.89	-	-	39.55	-	-	-
20	5.25	-	32.77	32.78	36.54	-	-	-	-	-	-
16	5.25	-	27.2^*	31.26	37.15	38.77^*	-	-	-	-	-
11	6.25	-	30.49	32.01	35.84	39.79	37.56	-	-	-	-
9	6.25	-	27.08^*	35.63	36.3	38.74	-	-	-	-	-
7	6.25	-	34.51	35.22	37.93	-	-	-	-	-	-
70	6.25	-	27^*	30.42	33.61	33.16	33.88	27.27	-	-	-
5	6.25	-	37.27	-	37.28	-	-	-	-	-	-

Inocula doses expressed as Log₁₀EID₅₀/100µl. No bird inoculated with lower viral titers became infected based on viral excretion and seroconversion (not shown).

Ct values are from RT-PCR analyses using avian influenza H5 specific primers and probe. Samples with no Ct values are shown with -.

Indicates that RT-PCR positive cloacal swabs were detected on the DPI shown.

Presence of viable virus confirmed by virus isolation in embryonating egg culture.