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. Author manuscript; available in PMC: 2012 Aug 22.
Published in final edited form as: J Neurosci. 2012 Feb 22;32(8):2683–2695. doi: 10.1523/JNEUROSCI.4125-11.2011

Figure 6.

Figure 6

Neurabin scaffolds formation of the A1R-RGS4 complex. A, The A1R-RGS4 interaction is dependent upon agonist stimulation and the presence of neurabin. Cells co-expressing HA-A1R and RGS4, together with or without Myc-Nrb, were incubated in the presence or absence of R-PIA for 5 min. Cell lysates were subjected to the immunoisolation assay and analyzed using an anti-HA antibody. Representative blots of at least three independent experiments are shown. B, RGS4 forms a complex with neurabin and the A1R upon agonist stimulation. Cells co-expressing HA-A1R, RGS4 and Myc-Nrb were incubated with or without R-PIA for 5 min. Cell lysates were subjected to the immunoisolation assay using an anti-RGS4 antibody and analyzed by Western blot. Representative blots of multiple independent experiments are shown. C, R-PIA stimulation fails to promote interaction between neurabin and RGS2 in cells. Cells co-expressing A1R, Myc-Nrb and RGS2 were stimulated with R-PIA for 5 min. Cell lysates were subjected to immunoisolation assay using an anti-RGS2 antibody and analyzed by Western blot. Representative blots of multiple independent experiments are shown.