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. Author manuscript; available in PMC: 2012 Sep 7.
Published in final edited form as: J Neurosci. 2012 Mar 7;32(10):3516–3528. doi: 10.1523/JNEUROSCI.3905-11.2012

Figure 3.

Figure 3

The glucocorticoids dexamethasone and prednisolone enhance hair cell regeneration. A. Fluorescent confocal images of GFP+ hair cells treated continuously for 48 hrs with DMSO vehicle (left), 5 µM dexamethasone (middle) or 5 µM prednisolone (right), after 400 µM neomycin treatment for 1 hr. Scale = 2.5 µm. B, C. An increase in total number of hair cells occurs following exposure to either dexamethasone (B) or presdnisolone (C). Graphs indicate the mean total number of GFP+ hair cells among seven neuromasts from 10 fish after neomycin damage (triangles) or mock treatment (squares) and subsequent 48 hr exposure to dexamethasone or prednisolone (denoted by open symbols and dotted lines in comparison to DMSO vehicle-only denoted with filled symbols and solid lines. Analysis by 2-way ANOVA showed significant main effects of drug, concentration and an interaction for dexamethasone and prednisolone as compared to DMSO control fish in both neomycin and mock-treated groups. Error bars indicate +/− 1 SD.