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Time-dependent effect of LPS and DOM on rat neonatal microglia TNF-α release. Rat neonatal microglia (2.8-5.0 × 106 cells/culture dish) were treated with LPS [3 ng/mL] or DOM [1mM] for 4 to 24 hours. TNF-α was determined as described in Experimental. Data (pg/mL) show the mean ± SE of 4-9 independent experiments. ** P < 0.01 vs. vehicle control.
