Skip to main content
. Author manuscript; available in PMC: 2008 May 5.
Published in final edited form as: Mar Drugs. 2007 Sep;5(3):113–135. doi: 10.3390/md503113

Figure 8.

Figure 8

RT-PCR analysis of MMP-9 gene expression in rat neonatal microglia after in vitro treatment with LPS or DOM. Rat neonatal microglia (2.8-5.0 × 106 cells/culture dish) were treated with (A) LPS [3 ng/mL] or (B) DOM [1mM] for 4 to 24 hours as described in Experimental. Amplification of MMP-9 and S12 mRNAs showed the predicted cDNA size after separation on a 1.5 % agarose gel and visualization by SYBRR Gold nucleic acid staining. The S12 ribosomal RNA gene product was amplified for 25 and 30 cycles and demonstrated equal loading of the gels. Quantification of the MMP-9 product was done after 25 cycles shown to be in the linear range. The figure depicts one of two similar experiments.