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The mean intensity of wide-field images, 〈iw〉, and standard deviation σm of five different fluorescent beads, obtained from experiment and theory.
| Experiment
|
Theory
|
||||
|---|---|---|---|---|---|
| Bead | 〈f〉 (cts) | σm (cts) | r | iw (cts) | r̂ |
| 1 | 1.70 × 105 | 836 | 4.09 | 5.01 × 105 | 4.11 |
| 2 | 1.20 × 105 | 669 | 3.90 | 4.13 × 105 | 4.38 |
| 3 | 10.29 × 104 | 511 | 3.24 | 2.32 × 105 | 3.56 |
| 4 | 1.89 × 105 | 750 | 3.50 | 5.04 × 105 | 3.85 |
| 5 | 9.05 × 104 | 526 | 3.54 | 2.80 × 105 | 4.15 |
| mean: | 3.66 | mean: | 4.01 | ||
