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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Antiviral Res. 2012 Mar 23;94(3):258–271. doi: 10.1016/j.antiviral.2012.03.005

Figure 4.

Figure 4

Quercetin added 6h after RV infection decreases viral load and replication. Cells were infected and treated with quercetin as described in Figure 3. Cells along with media was collected at 6 (just before adding quercetin) or 24 h (18 h after addition of quercetin or DMSO) after RV infection and infectious viral load was determined (A). Total RNA extracted from cells incubated for a total of 24 h post RV infection was used for determination of vRNA (B). The amplified qPCR product of negative strand RNA was subjected to agarose gel electrophoresis to show the amplified negative-strand vRNA (C). This result was representative of 3 experiments. (*p≤0.05, different from UV-RV in A and B; and different from sham in D; †p≤0.05, different from media or DMSO treated cells).