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. Author manuscript; available in PMC: 2012 Sep 21.
Published in final edited form as: J Neurosci. 2012 Mar 21;32(12):4196–4211. doi: 10.1523/JNEUROSCI.3094-11.2012

Figure 4. Ultrastructural analysis of fmi-1 synaptic defects.

Figure 4

A) Wild-type animals NMJs formed as a single active zone (arrow) surrounded by evenly sized, clear synaptic vesicles. Below a schematic is provided to illustrate the motorneuron (MN) and muscle (Mu). The active zone (black object) was surrounded by synaptic vesicles (grey circles), only some of the vesicles are illustrated for simplicity. B–D) fmi-1 mutants did have some normal NMJs (B). However, many fmi-1 animals also exhibited abnormal junctions (C,D). C) In this junction two neurons had vesicle filled swellings at the point of contact with the muscle; however, only one had an active zone (arrowhead). This is illustrated below where motorneuron 1 (MN1) has an active zone, but motorneuron 2 (MN2) does not, despite both having an accumulation of synaptic vesicles. D) Electron dense vesicles were present at ju43 NMJs (arrowhead), but not in wild-type animals (A). In the schematic the electron dense structures are illustrated as black circles. Scale bar is 100 nm.