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. Author manuscript; available in PMC: 2013 Nov 25.
Published in final edited form as: Cell Prolif. 2011 Jun 6;44(4):10.1111/j.1365-2184.2011.00757.x. doi: 10.1111/j.1365-2184.2011.00757.x

Figure 2. CXCL13:CXCR5 interaction promotes LNCaP and PC3 cell invasion independent of DOCK2.

Figure 2

LNCaP and PC3 cells were tested for their ability to invade Matrigel™ matrix and migrate through an 8.0 μm porous membrane in the presence of CXCL13 (100 ng/ml), isotype control antibody or anti-human CXCR5 antibody (1 μg/ml), DOCK2 or control siRNA (2 μM). Cells which invaded to the lower surfaces of the membrane were stained with crystal violet and counted using light microscopy at 40× magnification. Percentage cell invasion was calculated following manufacturer’s instructions (BD Biosciences). Error bars represent standard error of means of three independent experiments. *Significant differences (P < 0.05) relative to CXCL13-treated cells (control).