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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Cell Mol Immunol. 2012 Apr 23;9(4):341–349. doi: 10.1038/cmi.2012.13

Figure 5.

Figure 5

CD28 ligation increases the number of peritoneal cavity IFNγ-secreting cells. For Panel A, 0.5 and 1.0 × 105 C57BL/6J PerC cells +/− 1-MA were cultured with anti-CD3 +/− anti-CD28 as described in Methods. For Panel B, 0.75 and 1.5 × 105 C57BL/6J or BALB/c PerC cells were cultured with anti-CD3 +/− anti-CD28. For Panel C, 1.0 and 1.5 × 105 C57BL/6J PerC cells alone or with a neutralizing anti-IL-10 mAb were cultured with anti-CD3 +/− anti-CD28 costimulation. All CI values represent the ratio of the costimulated response to the uncostimulated response. * = p < .05; ** p < .005. The data shown are representative of three separate experiments that generated similar results.