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. Author manuscript; available in PMC: 2013 May 3.
Published in final edited form as: J Neurosci. 2012 Apr 4;32(14):4762–4772. doi: 10.1523/JNEUROSCI.3560-11.2012

Figure 4. Astrocytes develop within cortical columns and maintain their spatial relationship through adulthood.

Figure 4

At E18, columns of radially arrayed projection neurons were visible in cortex (A,B). By P1, GFP+ cells with morphologies resembling immature astrocytes (pseudocolored in red) were visible within the columns (C,D). At P10, mature astrocytes (pseudocolored in blue in F) were found within columns distributed through the depth of cortex, much like they are in adult mice (E,F). Columns in 2 month old adult mice contained pyramidal neurons and multiple clusters of multiple astrocytes (G,H). GFP+ astrocytes were still found closely arrayed with columns of GFP+ cortical neurons in 10 month old mice, indicating that astrocyte addition and/or turnover in the adult mouse does not appear to result in the widespread dispersion of astrocytes. Scale bars: 100 μm (A-C, E, G-J) and 25 μm (D, F). Mature astrocytes are pseudocolored in blue in E, H and J. An immature astrocyte is pseudocolored in red in D. Arrows in A,C,E,G and I point towards the pial surface of the neocortex.