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. Author manuscript; available in PMC: 2013 May 3.
Published in final edited form as: J Neurosci. 2012 Apr 4;32(14):4762–4772. doi: 10.1523/JNEUROSCI.3560-11.2012

Figure 5. GFP+ cells express radial glia markers zebrin and nestin during development.

Figure 5

Between E16 and E18, GFP+ cells form columns of extending from the ventricles to the pia. GFP+ cells (green) near the ventricles in these columns expressed Nestin (A-D, red) or Zebrin (E-H, red), proteins expressed by radial glia. By P0, zebrin+/GFP+ cells were found in the parenchyma of cortex, away from the ventricular wall, presenting morphologies resembling a shortened radial glia A-D: arrows indicate GFP+/Nestin+ cells. E-L: Arrows indicate GFP+/zebrin+ processes and cells. A,E and F: Asterisks indicate the neocortical ventricular wall. Zebrin+/GFP+ cells were absent by P4, a time by which most radial glia have disappeared from the brain. Scale bars: 50 μm.