Table 5.

Criteria 2 and 3 for Spatial Working Memory Total Errors Z-Scores, Cumulative Life Stress, and Prefrontal Regions Of Interest; Dependent Variable: Spatial Working Memory Total Errors Z-Scores (Higher z-score = fewer errors)

Model	Variables	Unstandardized regression coefficients, Standard Error, p-value	F-change(df)	R-square change, p values
1	Pubertal Status	B=.009, SE=.112, p=.937	FΔ(2,41)=5.933	R2Δ=.224, p=.005
	Cumulative Life Stress	B= −.245, SE=.072, p=.002
2	Pubertal Status	B=.072, SE=.101, p=.481	FΔ (2,39)=8.037	R2Δ=.226, p=.001
	Cumulative Life Stress	B= −0.038, SE=.082, p=.647
	Frontal Gray Matter (Left Anterior Cingulate Cluster)	B= 1.37, SE=.532, p=.014
	Frontal White Matter (Right Prefrontal White Matter Cluster)	B=2.834, SE=.925, p=.004

Criterion 2 requires that the independent variable (cumulative life stress) is significantly associated with the dependent variable (working memory performance) in the absence of the mediator. Criterion 3 requires that the mediator (prefrontal regions of interest) has a significant unique effect on the dependent variable after adjusting for the independent variable, and the effect of the independent variable on the dependent variable is reduced upon the addition of the mediator to the model.