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Localization of the CPC proteins Survivin and Aurora B at kinetochores in metaphase MCF-7 cells is not affected by p190B deficiency. Representative confocal images of metaphase control and p190B deficient MCF-7 cells immunostained with antibodies to detect Survivin, Aurora B, or CREST anti-serum are shown. Fluorescence intensities of Survivin and Aurora B normalized to CREST are graphed. Data is representative of at least 30 cells per group quantified from 3 independent experiments.
