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. Author manuscript; available in PMC: 2012 Nov 26.
Published in final edited form as: J Neurosci. 2012 Apr 25;32(17):5973–5983. doi: 10.1523/JNEUROSCI.3873-11.2012

Figure 3.

Figure 3

Phospho-ERK expression in C4 phrenic motor neurons after intrathecal EPO injection. (A) Phospho-ERK (dark brown staining) is expressed in C4 phrenic motor neurons and is colocalized with CtB (upper row, vehicle). pERK is upregulated after intrathecal EPO injection (middle row) and this upregulation is blocked by U0126 (UO; MEK/ERK inhibitor; 2nd row from bottom) and the PI3K/Akt inhibitor LY294002 (LY; bottom row). Densitometry in CtB labeled phrenic motor neurons showed increased phospho-ERK after intrathecal EPO versus vehicle treated rats. Pre-treatment with U0126 and LY294002 brings pERK protein expression to vehicle levels. Data are means ± 1 SEM. * p < 0.05 versus all other groups. Scale bar, 100 μm.