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. Author manuscript; available in PMC: 2012 Nov 2.
Published in final edited form as: J Neurosci. 2012 May 2;32(18):6072–6080. doi: 10.1523/JNEUROSCI.6486-11.2012

Figure 4.

Figure 4

Loss of TH in VTA does not prevent the D1 mediated increase in glutamate transmission with AMPH stimulation. (A) Left: Confocal images from an AAV-THsiRNA and an AAV-SCR injected mice acquired at 5× represent mean fluorescence from each group. TH staining is observed in the VTA of AAV-SCR injected mice but is virtually absent in mice injected with AAV-THsiRNA. Magnification bar indicates 0.5mm. Right: Coronal drawing depicts bilateral location of VTA and surgical targeting adapted from reference mouse brain atlas: Allen Institute for Brain Sciences. (B) Plot shows AMPH increases glutamate transmission in slices from both AAV-THsiRNA and AAV-SCR injected mice (THsiRNA: 119±4%; n=4; SCR: 120±8%; n=6; p>0.05). (C) Plot shows SKF increases glutamate transmission in slices from AAV-THsiRNA injected mice (AAV-THsiRNA SKF: 135±1%; p>0.05; n=4). AMPH is replotted for comparison. Error bars represent SEM.