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. Author manuscript; available in PMC: 2013 Feb 8.
Published in final edited form as: J Vis Exp. 2012 Feb 8;(60):10.3791/3807 3807. doi: 10.3791/3807

Figure 5. Serial dilution for clonal parasite lines.

Figure 5

Four polyclonal populations (red, blue, yellow, green) are serially diluted in a single 384-well plate confluent with HFF cells. Row C and I receive 10 parasites per well and are 2-fold diluted (40 μl + 40 μl) until rows H and N, respectively. The shade of grey indicates the decreasing number of parasites per well. Typically, single clones are found in rows D-F and J-L.