Table of specific reagents and equipment:

Name of the reagent	Company	Catalogue number	Comments
ENU	Sigma-Aldrich	N3385	1 M Stock in DMSO, store at -20°C
EMS	Sigma-Aldrich	M0880	1 M Stock in DMSO, store at -20°C
Dextran Sulfate	Sigma-Aldrich	D4911
PDTC	Sigma-Aldrich	P8765	100 mM Stock in PBS
Diff Quick	EMD Chemicals	65044-93
Filter holder	Cole-Parmer	540100
3 μm polycarbonate filter	Whatman Schleicher & Schuell	110612
Hemocytometer	Hausser Scientific	1475
CO2 incubators	Various manufacturers		Humidified, 5% CO2, at 35, 37 and 40°C
Fluorescence microscope	Various manufacturers		Ideally inverted, wide-field with 63× or 100× oil objective

HBSSc (according to Black et al.¹¹):

•98.0 ml Hanks Balanced Salt Solution (Hyclone catalog number SH30588)

•100 μl 1M MgCl₂ (100 mM end)

•100 μl 1M CaCl₂ (100 mM end)

•2.0 ml 1M Hepes pH 7.3 (20 mM end)

•84 mg NaHCO₃ (10 mM end)