GR8a and GR66a are indispensible for l-canavanine induced nerve firings. A, Representative traces of l-canavanine evoked action potentials from control flies (w1118), Gr8a1, Gr66aex83, and mutants with rescue transgenes (Gr8a1;Gr8a-GAL4/UAS-Gr8a and Gr8a-Gal4/UAS-Gr66a;Gr66aex83). The recordings were performed from S5 sensilla. B, Tip recordings showing that GR8a and GR66a are required for l-canavanine induced nerve firings. The assays were performed from S3, S5, and S10 sensilla using animals of the indicted genotypes. n=10-12. The error bars represent SEMs. **P<0.01. C, Tip recordings showing l-arginine-induced action potentials in S3, S5 and S10 sensilla. n=10-11. The error bars represent SEMs. D, l-arginine induced action potentials in L4 and L6 sensilla. n=10-11. Shown are means ±SEMs.