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. Author manuscript; available in PMC: 2012 Dec 3.
Published in final edited form as: J Invest Dermatol. 2012 Jun 28;132(12):2735–2743. doi: 10.1038/jid.2012.205

Figure 1. Immunity Generated by Recombinant LJM11 Salivary Protein (rLJM11) is Polarized Toward a Th1 Response.

Figure 1

(a) HPLC purification of rLJM11 (↓). Inset: silver-stained gel of purified rLJM11. C57BL/6 mice were immunized with 500 ng of either rLJM11 or BSA in the left ear. (b) Total IgG levels against rLJM11 and the IgG2a:IgG1 ratio. IFN-γ, IL-10, and IL-4 production following in vitro stimulation of lymph node (LN, c) and (d) spleen cells with rLJM11. (e) DTH response in mice immunized with rLJM11 (■) or BSA (●). (f) The absolute number of CD4+ T cells per ear following sand fly challenge. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Data are representative of three independent experiments (n = 5).