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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Virus Res. 2012 May 19;167(2):247–258. doi: 10.1016/j.virusres.2012.05.006

Fig. 10. Effect of ectopically expressed EGFP-nsp15s on MHV replication.

Fig. 10

DBT cells were transfected with 100ng each plasmid DNA containing either the full-length or deletion mutant DI and DIII) nsp15s fused to EGFP as indicated at the bottom of the x-axis. Cells transfected with the vector plasmid expressing EGFP alone were used as a negative control. At 9 h post transfection, the cells were infected with MHV-2aFLS at m.o.i. of 5. Luciferase activity was determined at 14 h p.i., and was expressed as percent relative to the negative control, which is set as 100%. The results were analyzed for statistical significance between the testing and control groups using the Student’s t test. P values of <0.01 or <0.05 were considered statistically significant, which is indicated with an asterisk.