Figure 2. Lck is important in OGD PC neuroprotection.

A, Primary cortical neurons were incubated in glucose free Earl’s balanced salt solution under 5% CO₂/95% N₂ at 37°C for oxygen glucose deprivation (OGD). OGD PC (30 min) reduced lethal OGD (2 hr)-induced neuronal cytotoxicity in PI-staining. OGD PC was performed 24 hr prior to lethal OGD stimulation. Cytotoxicity was examined at 24 hr after lethal OGD stimulation. B, Silencing Lck significantly reversed OGD PC neuroprotection. siRNAs were transfected 48 hr prior to OGD PC, and OGD PC/lethal OGD exposure was performed as described above. *p<0.05 and **p<0.01 vs. OGD lethal; #p<0.05 vs. Neg+OGD PC+OGD lethal. C, Protective effect of OGD PC was not found in primary neurons from Lck^−/− mice. D, Lck kinase activity was increased during OGD PC. Neuronal lysates were collected after OGD PC, and used for Lck immunoprecipitation and kinase assay. Total level of Lck in each immunoprecipitates was determined by western blot. *p<0.05 vs. Control without PC. A:n=4, B:n=3, C:n=5, D:n=3. All values are means ± SEM and analyzed by Student’s t-test.