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Indentation stiffness (A, B, E, F) and 45Ca++ uptake (C, D, G, H) by AC (A, C, E, G) and GPC (B, D, F, H) as a function of tissue layer and culture duration up to 3 weeks, n = 6–8 (A-D) and as a function of tissue layer, cell viability, and media supplementation with βGP after incubation for 3 weeks, n = 6–8 (E-H). Significant differences from t=1wk (A-D) or from live tissue with 10 mM βGP addition (E-H) are indicated by a (p<0.05), b (p<0.01), c (p<0.001).
