Table 6.
Recognition of VAC-Kd-infected Ewing's sarcoma cell lines by vaccinia-specific H-2Kd-restricted murine CTL
| Cytotoxicity (% lysis) with effectors: |
||||||
|---|---|---|---|---|---|---|
| Cell line | Vaccinia infectiona | IFNγb | E:T | CTL-1 | CTL-2 | TIL 888 |
| TC-71 | VAC-WT | − | 40:1 | −4 | −2 | −5 |
| 10:1 | −1 | −1 | −3 | |||
| + | 40:1 | −1 | 9 | 7 | ||
| 10:1 | 4 | 14 | 12 | |||
| VAC-Kd | − | 40:1 | 34 | 18 | −3 | |
| 10:1 | 17 | 15 | 2 | |||
| + | 40:1 | 23 | 18 | 3 | ||
| 10:1 | 19 | 17 | 2 | |||
| RD-ES | VAC-WT | − | 40:1 | 2 | 2 | 0 |
| 10:1 | 7 | 1 | 4 | |||
| + | 40:1 | 0 | 2 | 5 | ||
| 10:1 | 1 | 3 | 5 | |||
| VAC-Kd | − | 40:1 | 22 | 5 | −13 | |
| 10:1 | 11 | −3 | −8 | |||
| + | 40:1 | 28 | 21 | −2 | ||
| 10:1 | 20 | 13 | 3 | |||
| 6647 | VAC-WT | − | 40:1 | −1 | 2 | −1 |
| 10:1 | 1 | 4 | 1 | |||
| + | 40:1 | 4 | 4 | 7 | ||
| 10:1 | 3 | 5 | 5 | |||
| VAC-Kd | − | 40:1 | 21 | 16 | −2 | |
| 10:1 | 18 | 11 | 12 | |||
| + | 40:1 | 36 | 30 | −3 | ||
| 10:1 | 29 | 15 | 0 | |||
| T2 | VAC-Kd | − | 40:1 | 1 | −2 | −2 |
| 10:1 | 4 | −1 | 1 | |||
| + | 40:1 | −2 | −3 | −3 | ||
| 10:1 | 1 | 0 | 2 | |||
| 888-mel | None | − | 40:1 | −2 | −1 | 40 |
| 10:1 | 2 | 1 | 29 | |||
Results are from a 4-h 51Cr-release assay after infection of targets with appropriate vaccinia construct
a
VAC-WT, vaccinia virus wild type; VAC-Kd, vaccinia virus H-2Kd construct
b
IFNγ 500 U/ml for 72 h