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. Author manuscript; available in PMC: 2012 Dec 13.
Published in final edited form as: J Neurosci. 2012 Jun 13;32(24):8127–8137. doi: 10.1523/JNEUROSCI.6034-11.2012

Figure 6.

Figure 6

BDNF induces surface delivery of newly translated GluA1. A, Immunoblot of hippocampal total cell lysates following a 10 min puromycin (10 ng/ml) pulse followed by BDNF stimulation (40 min) in the absence or presence of anisomycin (Aniso, 10 μM) and probed with puromycin or β-tubulin antibodies. B, Western blot showing levels of surface biotinylated GluA1 (streptavidin), and puromycin incorporation (puromycin) from hippocampal lysates immunoprecipitated with an antibody to GluA1 following BDNF stimulation in the absence or presence of anisomycin. β-tubulin was probed from accompanying total cell lysates. C, Quantification of the ratio of surface biotinylated GluA1 and puromycin incorporation to β-tubulin for indicated conditions (n = 5 from 5 independent experiments). Error bars indicate SEM. *p < 0.05 by Student's t test.