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. Author manuscript; available in PMC: 2012 Dec 20.
Published in final edited form as: J Neurosci. 2012 Jun 20;32(25):8532–8544. doi: 10.1523/JNEUROSCI.0337-12.2012

Figure 4. CCh and M1 compound VU0364572 induce β-arrestin recruitment in TREx CHO cells.

Figure 4

(a.) Pre agonist and post agonist confocal scans of hM1 TREx CHO cells expressing β-arrestin2-YFP. Treatment of cells with CCh (100 μM) induces β-arrestin recruitment (black arrows, bottom panel) in cells that were exposed overnight to TET (50 ng/mL). Treatment of cells with VU0364572 (100 μM) induces β-arrestin2 recruitment (black arrows, bottom panel) in cells that were exposed overnight to TET (1 μg/mL). VU0357017 (100 μM) did not induce β-arrestin2 recruitment in cells treated overnight with 50 ng/mL or 1 μg/mL TET. (b.) Quantification of the effects of each agonist on the number of puncta. A one-way ANOVA revealed that puncta in CCh treated cells (50 ng/mL TET) differed significantly when compared to VU0357017 (1 μg/mL TET) and VU0364572 (1 μg/mL TET) treated cells (F (3, 26) = 4.82, p < 0.001). Neither CCh nor either M1 agonist induced arrestin recruitment in cells that were not treated with TET (data not shown).