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. Author manuscript; available in PMC: 2012 Dec 20.
Published in final edited form as: J Neurosci. 2012 Jun 20;32(25):8532–8544. doi: 10.1523/JNEUROSCI.0337-12.2012

Figure 5. M1 agonists VU0364572 and VU0357017 significantly enhance threshold Θ-burst LTP and VU0364572 induces LTD at the Schaffer collateral-CA1 synapse of rodent hippocampal slices.

Figure 5

Insets for each figure are representative fEPSP traces measured at baseline (black) or 50 (LTD) or 55 (LTP) minutes after compound washout (gray). Scale bars are x-axis = 2ms, y-axis = 0.6mV. (a.) The standard TBS protocol (TBS-saturation) induces significant LTP (n=9) whereas the threshold TBS protocol induces only a slight potentiation of fEPSP slope (n=8) at the SC-CA1 synapse. (b.) Bath application of 500 nM VU0364572 (n=5) or VU0357017 (n=5 out of 9 experiments) for 10 minutes prior to threshold TBS induced a significant potentiation of fEPSP slope. (c.) Significant differences (P < 0.01) were observed in the mean percent potentiation induced by threshold TBS compared to TBS-saturation, or TBS-threshold plus compound. (d.) Addition of 50 μM (n=5) CCh for 10 minutes induced LTD of fEPSP slope whereas addition of 30 μM CCh (n=4) had no effect. (e.) Bath application of 30 μM VU0364572 (n=6) for 10 minutes induced LTD, whereas addition of 30 μM VU0357017 (n=6) had no effect on LTD. (f.) Mean percent maximal depression induced by each compound. VU0364572 induced a significant depression in fEPSP slope compared to 30 μM CCh.