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IL-12 modulated the expression of CD62L on in vitro activated OT-1 cells. OT-1 cells primed with or without 10 ng/ml of IL-12 were cultured for 3 days and sorted into CD8+ CD62Llo/sham, CD8+ CD62Lhi/sham, CD8+ CD62Llo/IL-12 or CD8+ CD62Lhi/IL-12 (a). Sham-treated (b) or IL-12-treated (c) cells were re-cultured with or without IL-12 at a cell density of 1 × 106/ml. Expression of CD62L was analyzed by flow cytometry at days 5 and 7 after priming (days 2 and 4 after reculture)
