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. Author manuscript; available in PMC: 2012 Dec 27.
Published in final edited form as: J Neurosci. 2012 Jun 27;32(26):9066–9072. doi: 10.1523/JNEUROSCI.0868-12.2012

Figure 2. Control experiments demonstrate that modulation of the Ih is caused by activation of muscarinic acetylcholine receptors and is not caused by wash-out.

Figure 2

A. Ih tail currents were analyzed in control ACSF before a delay (blue) and in the same control ACSF after a 15 minute holding period (green) as shown in this example. B. Averages across all cells demonstrate no significant change in the Ih tail current magnitude after application of carbachol. C. Ih tail currents were analyzed in atropine (blue) and in atropine with carbachol (green). D. Averages across all cells demonstrate no significant change in the Ih tail current magnitude after application of carbachol with atropine. E. The tail current protocol was used in control and after bath application of ZD7288. F. After application of ZD7288 the tail current amplitude is completely abolished.