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. Author manuscript; available in PMC: 2013 Jan 25.
Published in final edited form as: J Neurosci. 2012 Jul 25;32(30):10226–10237. doi: 10.1523/JNEUROSCI.0007-12.2012

Figure 5.

Figure 5

Transmitter phenotypes of α6(+) neurons in superior colliculus. A. α6* nAChRs are expressed in GABAergic neurons in sSC. Coronal sections from α6-GFP transgenic mice containing sSC were double stained with anti-GFP and anti-GAD67 antibodies, followed by confocal microscopy. A2 represents the boxed area in the A1 image. Single white arrows indicate cells co-expressing α6* nAChRs and GAD67, whereas double white arrows indicate cells expressing only GAD67. B. α6* nAChRs are also expressed in glutamatergic fibers in sSC. Coronal sections from α6-GFP transgenic mice containing sSC were double stained with anti-GFP and anti-VGLUT1 antibodies, followed by confocal microscopy. B2 represents the boxed area in the B1 image. White arrows indicate co-localized VGLUT1 and α6* nAChRs. C. Intermediate superior colliculus (iSC)α6(+) neurons are not GABAergic. Coronal sections from α6-GFP transgenic mice containing iSC were double stained with anti-GFP and anti-GAD67 antibodies, followed by confocal microscopy. C2 represents the boxed area in the C1 image.