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. Author manuscript; available in PMC: 2012 Nov 18.
Published in final edited form as: J Immunol. 2012 Sep 5;189(7):3681–3688. doi: 10.4049/jimmunol.1201541

Figure 4. TLR4 deficiency promotes Th17/Treg imbalance in atherosclerotic lesions following infection with P. gingivalis.

Figure 4

Quantitative immunohistochemistry of (A) CD4, CD8, IL-17 positive staining area in the innominate artery of uninfected and P. gingivalis infected ApoE−/− and ApoE−/−TLR4−/− mice using ImageJ software (NIH) or (B) Foxp3 positive cell count. Three sections from n=4 mice/group were analyzed. White bar: Uninfected. Gray bar: P. gingivalis infected. Data are mean ± SD of positive staining area/slice or cell count/slice. Intra-genotype comparisons were calculated by Mann Whitney U test ***p<0.0001. NS= not significant. Inter-genotype comparisons were calculated by Two-way ANOVA (indicated p values). (C) Representative immunohistochemistry of CD4, CD8, IL-17, and Foxp3 positive cells in the innominate artery of uninfected and P. gingivalis infected ApoE−/− and ApoE−/−TLR4−/− mice. Scale=5μm.