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. Author manuscript; available in PMC: 2012 Nov 18.
Published in final edited form as: J Immunol. 2012 Sep 5;189(7):3681–3688. doi: 10.4049/jimmunol.1201541

Figure 6. Impaired Th1 immunity and Th17 skewing in P. gingivalis infected TLR4-deficient mice.

Figure 6

Expression of IFN-γ and IL-17 in (A) CD4+ and (B) CD8+ splenic T cells from uninfected and P. gingivalis infected mice following in vitro stimulation with 10 μg/ml P. gingivalis soluble antigens and 1 μg/ml anti-mouse CD28 for 4h in the continuous presence of Brefeldin A. Data represent mean ± SD, n=4/group. White bar= uninfected, Gray bar= P. gingivalis infected. Intra-genotype comparisons were calculated by Mann Whitney U test. **p<0.01, ***p<0.001. Inter-genotype comparisons were calculated by Two-way ANOVA with Bonferonni’s post-test. (C) Representative flow cytometry gating for the analysis of cytokine secreting CD4+ and CD8+ T cells.