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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Neurosci. 2012 Aug 1;32(31):10587–10593. doi: 10.1523/JNEUROSCI.0015-12.2012

Fig 2.

Fig 2

FRAP of RHO and IFT88 in cilia of hTERT-RPE1 cells. (A) Cells cotransfected to express either RHO-EGFP with ARL13B-mCherry or IFT88-YFP with ARL13B-mCherry. ARL13B-mCherry was used as a live-cell cilium marker; right panels show shifted overlays. Scale bar = 5μm. (B) Images illustrating fluorescence recovery of RHO-EGFP and IFT88-YFP in the distal cilium. Pre, Pre-bleach; Post, recovery 3 minutes after photobleaching. Scale bar = 1 μm. (C, D) Examples of plots of normalized fluorescence recovery in the distal cilium vs time.