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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Neurosci. 2012 Aug 1;32(31):10587–10593. doi: 10.1523/JNEUROSCI.0015-12.2012

Fig 5.

Fig 5

Reduction in FRAP of RHO-EGFP in the connecting cilium of Kif3a−/− mouse rod photoreceptor cells. (A) Example of plot of normalized fluorescence recovery vs time for RHO-EGFP in the connecting cilia of Kif3a+/+ and Kif3a−/− rod photoreceptors (full genotypes: RHO-EGFP(KI);Rpe65−/−;RHO-Cre+ and RHO-EGFP(KI);Rpe65−/−;Kif3a flox/flox; RHO-Cre+, respectively). (B) Bar graph of the measured half-times, t1/2, for recovery of RHO-EGFP in Kif3a+/+ and Kif3a−/− rod photoreceptors. Kif3a+/+;Cre+ represents RHO-EGFP(KI);Rpe65−/−;RHO-Cre+ (i.e. lacking the floxed gene, but with the Cre transgene). Measurements of t1/2 were 11.2 ± 1.7, 11.4 ± 2.7, and 37.4 ± 6.8 secs, for Kif3a+/+ (n=42), Kif3a+/+;Cre+ (n=10), and Kif3a−/− (n=24), respectively (p<0.0001 for Kif3a+/+ vs Kif3a−/−, p<0.01 for Kif3a+/+;Cre+ vs Kif3a−/−, p=0.96 for Kif3a+/+ vs Kif3a+/+;Cre+).