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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: J Neurosci. 2012 Aug 15;32(33):11343–11355. doi: 10.1523/JNEUROSCI.1436-12.2012

Fig. 4. Gross morphology of all cell classes is normal in the Gnb3-null retina.

Fig. 4

Immunostaining using several cell markers for wild type (WT, top) and Gnb3-null (KO, bottom) retinas. Recoverin labels all photoreceptors throughout the cell including outer and inner segments, somas and photoreceptor terminals. All these segments are stained in both wild type and null retinas. Calbindin labels horizontal cells strongly and certain amacrine cells weakly. Horizontal cells and the three bands in the IPL (arrows) appear similar. Synaptophysin staining shows that the two synaptic layers are similar. Choline acetyl transferase (Chat) labels the cholinergic amacrine cells which stratify in the OFF (top band) and ON sublaminas (lower band) of the IPL; these bands are unaffected by the absence of Gβ3. Data are representative of 3 (synaptophysin) to 8 (calbindin) images from 3 different animals for each genotype.