Fig. 9. Gβ3 rather than Gβ5 forms the heterotrimer Go.
A. Immunostaining in wild type (top) and Gβ5-null (bottom) retinas for Gαo, Gβ3, Gγ13, and TRPM1. Fixed retinas and sections were obtained from Dr. Chen. B. Relative staining intensity (as defined in Fig. 6C) for Gαo, Gγ13, and TRPM1 in several retinal layers and their sum. For TRPM1, number of puncta were calculated as in previous figures. Gβ3 staining was not quantified because staining only worked on one set of tissues. However, results in the Chen lab showed similar staining intensity in wild type and null tissues. Average ratios represent results from at least 3 sets of experiments. C. Co-immunoprecipitation using a monoclonal antibody to pull down Gαo and its interacting proteins. Top blot was probed for Gβ3 and bottom one for Gαo. The relevant lanes, i.e., immunoprecipitated (IP) protein with and without the antibody, are boxed with dotted squares. H, homogenate; F1 and F2 flow through. D. Co-immunoprecipitation samples probed for Gβ5.