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. Author manuscript; available in PMC: 2013 Feb 22.
Published in final edited form as: J Neurosci. 2012 Aug 22;32(34):11716–11726. doi: 10.1523/JNEUROSCI.1942-12.2012

Figure 4. Stimulation of NMDA-Rs in hippocampal neurons triggers a conformational change in BRAG1.

Figure 4

(A–C) Frames from time-lapse movies of DIV 12 hippocampal neurons expressing mCherry-tagged BRAG1 constructs and treated with 30 μM NMDA for the times shown. (A) BRAG1-WT, (B) BRAG1-IQ, (C) BRAG1-ΔN. Arrowheads indicate dendritic spines where single BRAG1 foci resolve into distinct puncta. Arrows indicate formation of new puncta in the dendritic shaft (D) Quantitation of puncta formation. Mean number of puncta per 100 μm2 pre- and post-NMDA stimulation (WT-pre: 17.5±1.4 puncta; WT-post: 46.1±6.6 puncta; n=3; p<0.05; IQ-pre: 20.0±3.4 puncta; IQ-post: 20.5±2.4 puncta; n=4; p=0.5; ΔN-pre: 6.0±4.1 puncta; ΔN-post: 7.0±3.9 puncta; n=3; p=0.5). Asterisk indicates p ≤ 0.05 (Wilcoxon). Scale bars = 10 μm.