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. Author manuscript; available in PMC: 2013 Feb 22.
Published in final edited form as: J Neurosci. 2012 Aug 22;32(34):11716–11726. doi: 10.1523/JNEUROSCI.1942-12.2012

Figure 7. BRAG1-WT and BRAG1 mutants differentially affect synaptic transmission.

Figure 7

(A) Evoked AMPA-R- (−60 mV) and NMDA-R- (+40 mV) mediated responses recorded from neighboring non-expressing (Ctrl) and mCherry-tagged BRAG1-WT or BRAG1-ΔN expressing neurons cultured in normal media or media containing 100 μM DL-APV. (B) Values for AMPA responses in neurons expressing BRAG1-WT and BRAG1-ΔN cultured in normal media (Ctrl: −31.6±2.5 pA; WT: −22.9±2.4 pA; n=38; p<0.05; Ctrl: −14.2±2.1 pA; ΔN: −20.1±3.2 pA; n=16; p<0.05), in media containing high Mg2+ (Ctrl: −26.8±2.6 pA; WT: −28.8±3.1 pA; n=22; p=0.94; Ctrl: −20.3±1.7 pA; ΔN: −21.4±2.3 pA; n=23; p=0.93), or in media containing DL-APV (Ctrl: −31.7±2.3 pA; WT: −30.1±2.4 pA; n=19; p=0.51; Ctrl: −22.3±3.8 pA; ΔN: −23.8±2.5 pA; n=19; p=0.69), and for NMDA responses in neurons expressing Brag1-WT and BRAG1-ΔN cultured in normal media (Ctrl: 79.3±6.5 pA; WT: 75.9±6.8 pA; n=38; p=0.60; Ctrl: 46.6±9.7 pA; ΔN: 41.3±8.5 pA; n=16; p=0.33), in media containing high Mg2+ (Ctrl: 66.4±11.6 pA; WT: 61.6±9.4 pA; n=22; p=0.78; Ctrl: 53.8±5.3 pA; ΔN: 54.3±7.4 pA; n=23; p=0.72), or in media containing DL-APV (Ctrl: 59.2±7.3 pA; WT: 59.3±6.7 pA; n=19; p=0.94; Ctrl: 47.4±6.8 pA; ΔN: 44.4±5.5 pA; n=19; p=0.57) related to control non-expressing neurons. (C) Evoked AMPA-R- (−60 mV) and NMDA-R- (+40 mV) mediated responses recorded from neighboring non-expressing (Ctrl) with either mCherry-tagged BRAG1-EK or BRAG1-IQ expressing neurons cultured in normal media or media containing 100 μM DL-APV. (D) Values for AMPA responses in neurons expressing BRAG1-EK and BRAG1-IQ cultured in normal media (Ctrl: -24.1±2.8 pA; EK: −26.5±2.5 pA; n=21; p=0.52; Ctrl: −25.4±2.6 pA; IQ: −19.4±1.9 pA; n=25; p<0.05), in neurons expressing BRAG1-IQ cultured in media containing high Mg2+ (Ctrl: −23.0±2.3 pA; IQ: −15.4±1.3 pA; n=25; p<0.005) or DL-APV (Ctrl: −26.7±2.8 pA; IQ: −19.9±1.7 pA; n=24; p<0.01), and for NMDA responses in neurons expressing BRAG1-EK or BRAG1-IQ cultured in normal media (Ctrl: 77.1±9.1 pA; EK: 74.1±8.0 pA; n=21; p=0.54; Ctrl: 52.5±5.2 pA; IQ: 50.8±6.6 pA; n=25; p=0.14), in neurons expressing BRAG1-IQ cultured in media containing high Mg2+ (Ctrl: 57.6±5.7 pA; IQ: 52.9±5.7 pA; n=25; p=0.20) or DL-APV (Ctrl: 68.9±6.8 pA; IQ: 64.4±5.8 pA; n=24; p=0.67) related to control non-expressing neurons. The relative values and standard errors were normalized to average amounts of AMPA and NMDA responses from control CA1 cells. Asterisk indicates p<0.05 (Wilcoxon test).