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. Author manuscript; available in PMC: 2013 Feb 28.
Published in final edited form as: J Neurosci. 2012 Aug 29;32(35):12051–12065. doi: 10.1523/JNEUROSCI.1857-12.2012

Figure 3. Conditional dab1 deletion induces aberrant DGC migration.

Figure 3

A, C) NestinCreERT2/Dab1+/+ mice treated with tamoxifen (TMX) at P7-8 had properly located neuroblasts in the dentate subgranular zone and inner granule cell layer (gcl) as assessed by PSA-NCAM immunoreactivity 52 days later. In NestinCreERT2/Dab1flox/flox mice treated with the same TMX regimen, PSA-NCAM immunolabeling was more disorganized and many PSA-NCAM-positive cells appeared in the hilus (h; B, D). In addition, chains or clusters of presumptive migrating neuroblasts extended from the gcl into the hilus (arrowheads in D). Scale bar (in A), 50 μm for A–B, 35 μm for C–D. E–F) GFAP immunostaining of DG from P60 NestinCreERT2/Dab1+/+ control (E) and NestinCreERT2/Dab1flox/flox mice (F) treated with TMX at P7-8. Labeled glia, including radial glia-like cells in the gcl, appeared unaffected by conditional loss of dab1. h, hilus. Scale bar (in E), 150 μm.