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. Author manuscript; available in PMC: 2008 Apr 3.
Published in final edited form as: J Neurosci. 2005 Mar 23;25(12):3041–3045. doi: 10.1523/JNEUROSCI.5277-04.2005

Figure 1.

Figure 1

Expressions of nAChR subunits in SGNs. Expression of nAChR α2, α4 –7, and β2–3 subunits in SGNs were detected by RT-PCR, and samples from two mice of each age group (4 and 8 months of age) are shown (A). Expression of the neuronal marker snap-25 (internal control) is shown from two mice from each age group (B), and α5 and β2 protein products detected by Western blots are also shown from two mice (C). Levels of nAChR subunit mRNAs from each age group (6 mice per group) were quantified relative to snap-25 mRNA by RT-PCR (D). Expression levels of α4, α5, and β2 subunit mRNAs are significantly different between the two age groups (t test; *p< 0.01). Level of β2 subunit mRNA was compared further between 4-and 8-month-old groups (4 mice per group) from C57BL/6J and B6.CAST by real-time RT-PCR (E). Expression level of β2 subunit mRNA is significantly different between the two age groups for both C57BL/6J and B6.CAST (t test; *p<0.01). Error bars represent mean±SD.