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. Author manuscript; available in PMC: 2013 Mar 5.
Published in final edited form as: J Neurosci. 2012 Sep 5;32(36):12337–12348. doi: 10.1523/JNEUROSCI.2129-12.2012

Fig. 7. Differential pre- and postsynaptic changes inα7 nAChR-dependent LTP and STD revealed with dual-color GECI imaging.M.

Fig. 7

GECIs (green GCaMP3 and red R-GECO1) were expressed separately in hippocampal CA3 and CA1 to monitor pre- and postsynaptic activities simultaneously; (A to D) for LTP and (E to G) for STD. (A) Normalized presynaptic GCaMP3 responses showing smaller enhancement at 30 min than that at 10 min. (B) Normalized postsynaptic R-GECO1 responses showing stronger enhancement at 30 min than that at 10 min. (C) Bar graph showing the differential changes of the later stage (at 30 min) between pre- and postsynaptic activities during the LTP. (D) Images of pre- and postsynaptic SC stimulation-induced calcium increases at different time points during the LTP. Left two panels show the active pre- and postsynaptic calcium responses to SC stimulation, respectively. The third panels are merged images of pre- and postsynaptic responses, with the right panels showing some potential spines. (E) Normalized presynaptic GCaMP3 responses showing the transient depression after the SO pathway pairing protocol. (F) Normalized postsynaptic R-GECO1 responses showing the transient depression after pairing the SO pathway. (G) Bar graph showing the differential recovery between pre- and postsynaptic activities during the STD.