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. Author manuscript; available in PMC: 2013 Mar 5.
Published in final edited form as: J Neurosci. 2012 Sep 5;32(36):12421–12430. doi: 10.1523/JNEUROSCI.6381-11.2012

Figure 7.

Figure 7

(A) Noise trauma increases formation of Rac1/p67phox complexes. Complexes of Rac1 with p67phox (a subunit of NADPH oxidase) were detected by immunoprecipitation (IP) with an antibody against Rac1, followed by immunoblotting (IB) with an antibody for p67phox. The membrane was then immunoblotted with a Rac1 antibody as a control for the amount of Rac1 in the immunoprecipitation assay. The band densities of complexes of Rac1/p67phox quantified as described in 'Materials and Methods’ section were stronger after noise treatment in comparison to the control group (p < 0.05; n = 3). (B) Levels of p67phox in the cochlear total protein extracts were detected by Western blot and showed no change (p > 0.05; n = 3). GAPDH serves as a loading control.