Fig. 8.
Mean counts × 105 of subgingival taxa detected in 30 periodontally healthy subjects during experimental gingivitis. Subgingival plaque samples were collected from the mesio-buccal surface of 4 randomly selected teeth at different time points. The baseline visit occurred 21 days after a preparation phase to achieve gingival health and minimum plaque accumulation (time 0). Days 3, 7, 10, 14, 21 correspond to different time points after interruption of oral hygiene practices (experimental gingivitis phase); and day 35 corresponds to the resolution of the experimental gingivitis. Counts of 40 subgingival species were measured for each sample using checkerboard DNA-DNA hybridization. Data were averaged within a subject and then averaged across subjects in each time point separately. The species were ordered and grouped according to the complexes described by Socransky et al. (337). Figure adapted from data from Lee et al. (199).