Figure 4. Overexpression of mGluR5 restores bicuculline-induced synaptic scaling down in Mecp2^{S421A;S424A/y} hippocampal neurons.

A–D) Representative whole-cell recording sample traces, average mEPSC waveforms, cumulative probability distribution, and quantification of mEPSC amplitudes and frequencies from cultured Mecp2^{S421A;S424A/y} and wild type hippocampal neurons (18–20 DIV) that were transfected with lentivirus expressing only DsRed and treated with either control solution or bicuculline (40 μM) for 48h (n=48–52 cells in each group). E–H) Representative whole-cell recording sample traces, average mEPSC waveforms, cumulative probability distribution, and quantification of mEPSC amplitudes and frequencies from cultured Mecp2^{S421A;S424A/y} and wild type hippocampal neurons (18–20 DIV) that were transfected with lentivirus expressing both mGluR5 and DsRed and treated with either control solution or bicuculline (40 μM) for 48h (n=48–52 cells in each group). I and J: Representative images and quantification of surface GluA2 immunoreactivity in cultured Mecp2^{S421A;S424A/y} and wild type hippocampal neurons (18 DIV) that were transfected with DeRed or DsRed-Grm5 containing lentivirus and treated with either control solution or bicuculline (40 μM) for 48h. Scale bar:10 μm. (n=20 cells in each group). The bar graph shows the mean ± s.e.m * p<0.05. ** p<0.01.