Table 2.
Experimentally validated Eyes Absent binding proteins. [86]
| EYA-binding partner | Validation Method | Functional outcome | References |
|---|---|---|---|
| SIX/So and DACH/Dac | YTH, Genetic, IP | Nuclear translocation of EYA by SIX; increase of SIX transcriptional activity by EYA; Alleviation of DACH repressor activity toward SIX; Increase of SIX-DNA binding affinity | [25,8,86,154] |
| G z and G i | YTH, IP, IF | Recruitment of EYA to plasma membrane and prevention of SIX-mediated nuclear translocation | [155,46] |
| SIPL1and RBCK1 | YTH, IP | Enhancement of the transactivation potential of the SIX-EYA complex | [49] |
| aPKC-zeta | IP | Dephosphorylation of aPKC-zeta and NUMB, regulation of polarity in the lung epithelium | [50] |
| ATM/ATR | IP | Phosphorylation of EYA, interaction of EYA with H2AX | [22,51] |
| H2AX | IP, IF | Dephosphorylation of pY142- H2AX, initiation of DNA damage repair | [22,23] |
| Abl Kinase | IP, genetics | Phosphorylation of Eya and retention in the cytoplasm | [52] |
| Nemo Kinase | IP, genetics | Phosphorylation of Eya, potentiation of the transcriptional activity the Eya-So complex | [53] |
| IPS-1, STING, NLRX1 | IP | Dephosphorylation of a phospho-Ser/Thr substrate, enhancement of innate immune response | [17,44] |
| SOX2 | IP | Possibly acts with EYA1 in the generation of progenitor cells in the otocyst | [54] |
Abbreviations: IF, immunofluoresence; IP, immonuprecipitation; YTH, yeast-two-hybrid